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Sample Requirements
1.Cells (in vitro cultured cells or white blood cells, purified peripheral blood mononuclear cells (PBMCs): One to 5 million cells. When FACS-sorted and highly purified cells are provided, the number may be as low as 100,000 cells. We have performed TaqMan? analysis for gene transcription using blastocysts at the 32 and 64-cell stage with excellent results.The enough harvested cells stored in Trizol? could be shipped frozen (-20°C) in styrofoam boxes.The styrofoam boxes should be recycled.
2.Tissues: Fresh tissue should be provided in a frozen state on dry ice (stored at-80°C for up to 1 year). Please provide tissue samples at least 50-100 mg.The grinded sample stored in Trizol could be shipped frozen (-20°C) in styrofoam boxes.The styrofoam boxes should be recycled.
3.Fixed tissues: Two 50 micron sections of paraffin-embedded tissues are required from solid tissues. A third 50 micron section is required from non-solid tissues such as lung tissue. TaqMan? analysis were performed on a variety of paraffin-embedded tissues (i.e. skin, lymph node, GI tract, spleen, liver, lung, brain) which were up to 20 years of age without significant loss of PCR signals for the endogenous control (GAPDH,beta-atin or 18S rRNA) when compared to recently embedded tissue (within 1 year). |
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