Send IM to Anne
Send IM to Peter
 
  English | 中文
 
 
 
1
Taq Polymerase for qPCR
Taq antibody
Heat labile UNG
HotStart Taq Polymerase
Pfu DNA Polymerase
Kod DNA Polymerase
dNTPs
Reverse Transcriptase
RNasin
MiniRNA Prep
First Strand cDNA Synthesis Kit
Real-Time qPCR Kit
One Step qPCR Kit
DNA Marker
Lab Consumables
Lab Equipments
SARS-CoV-2
Sars-CoV-2 Spike RBD
Spin Column for  DNA/RNA
Silica Magnetic Bead
Proteinase K
8-Strip Tip Comb
Kingfisher 96 Deep Well Tip Combs
Pipette Tips With Filter
1

LinkedIn Google

PowerQ® Taq DNA Polymerase for qPCR

Catalog #

Pack size

Price()

ZP00102

1000U(5U/ul)

26.40

ZP00103

5000U(5U/ul)

106.40

Storage condition: The undiluted enzyme solution is stable when stored at -20°C.

Storage and dilution buffer: 20mM Tris-HCl; 1mM dithiothreitol; 0.1mM EDTA; 0.1M KCl; Nonidet P40, 0.5%(v/v); Tween 20, 0.5%(v/v); glycerol, 50%(v/v); pH 8.0 (4°C).

Concentration:5U/ul

Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10mmols of dNTP into an acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl, (pH 9.0 at 25°C), 50mM NaCl, 10Mm MgCl2, 200uM dATP, dCTP, dGTP and radiolabelled dTTP, and 12.5ug activated calf thymus DNA in a 50ul reaction.

10XReaction buffer(without MgCl2): 100mM Tris-HCl; 500mM KCl; pH 9.0 (20°C). Buffer is optimized for use with 0.2mM for each of dNTPs. A tube of 25mM MgCl2 is supplied separately.

Note: It is recommended to add dNTPs to this incubation mixture shortly before use. This is to prevent decomposition of the deoxynucleoside thiphosphate that occurs during Prolonged storage at the alkaline pH values required for optimal enzyme activity.

Quality control

Each lot of Taq DNA Polymerase is tested for activity in PCR and efficient incorporation of digoxigenin-11-dUTP, and in DNA sequencing of M13mp18ssDNA. A minimum of 250 bases must be clearly legible in the sequencing gel. Each lot of Taq DNA ploymerase is tested for contaminating activities as stated below.

Test buffer: 10mM Tris-HCl; 1.5mM MgCl2; 50mM KCl; pH 9.0 (20°C). 0.1mg/ml gelatin.

Absence of endonucleases: 1ul lambda DNA is incubated with Taq Dna polymerase in 50ul test buffer with a paraffin oil overlay at 65°C for 16 hours. The amount of enzyme showing no degradation of the lambda DNA is ststed under "Endol".
Taq DNA Polymerase
If you want to get bulk order price,please contact us.E-mail:master@shinegene.org.cn or shinegene@vip.163.com

LinkedIn Google

Related Link
Taq antibody
Heat labile UNG
One step RT-qPCR
RNasin
Peptide Synthesis
Chromas
Make Antibody
Lab Consumables
Gene Synthesis
dNTP


 

 

Copyright 2003-2020 Shanghai ShineGene Molecular Biotech,Inc. All Rights Reserved.
Floor 2,Gate 6,289#,Minqiang Road,Songjiang District,Shanghai 201612,China
Tel:0086-21-54460832    Fax:0086-21-54460831-13    E-mail:master@shinegene.org.cn