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SARS-CoV-2(2019-nCOV) Coronavirus and Influenza A/B
Virus Multiplex RT-qPCR Detection Kits
Only for Research!
Catalog # |
Pack size |
Price($) |
COV00102 |
50tests |
600.00 |
COV00103 |
100tests |
1000.00 |
This kit is used for in vitro diagnostics for 2019-Novel
Coronavirus (2019-nCoV) infection including suspected case,
suspected aggregative diseases and other scenarios. By
testing the suspected person’s respiratory specimen, serum
or blood, the kit can tell if the specimens contain any
virus RNA, so it can be used for clinical screening of
suspected cases.
Select the 2019-nCoV N gene and E gene as
the target amplifying area, design specific primer and
fluorescent probes, use FAM label, detect if the specimen
contains any 2019 nCoV RNA. Select the 2019-nCoV ORF1ab
gene, design specific primer and florescent probes, use ROX
label, detect if the specimen contains any 2019-nCoV RNA.
Select influenza A/B specific sequence, design specific
primer and fluorescent probes, use Cy5 label, detect if the
specimen contains any influenza A/B virus RNA. Select other
species’ sequence (none-human and none-targeted sequence) as
internal primer/probe, label with VIC, monitor the whole
process.
The kit also contains UNG enzyme
anti-contamination system. Its theory is it selectively
hydrolyzes Uracil glycoside bond in the single or double
chain of DNA that contains dU. This leads to a DNA chain
with nucleotide deletion which will further hydrolyzed in
alkaline media under high temperature and thus avoid
contamination by PCR amplification.
4
Fluorescent Signals and Target
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Fluorescent signal |
Target |
|
1 |
FAM |
2019-nCoV N gene and E gene |
|
2 |
ROX |
2019-nCoV ORF1ab gene |
|
3 |
Cy5 |
Influenza A/B specific sequence |
|
4 |
VIC |
MS2 internal standard (RNA Phage pseudo virus) |
Key
Features:
1.High sensitivity 500 copy/ml
2.Very specific, can detect 2019-nCoV E gene, N gene and
ORF1ab different zone sequence at the same time in one tube.
3.Can simultaneously identify and distinguish influenza
viruses to avoid misdiagnosis.
4.One-step RT-PCR, strong amplification signal, high
stability.
Storage condition:
-20°C.
Equipment Requirements:
Fluorescent PCR amplifier; Fluorescent PCR amplifier with 4
colors or multi-color fluorescent PCR amplifier such as
ABI Prism®7500, Bio-rad CFX96 etc.
Set up
reaction mixture:
|
Component |
Volume（μL） |
|
RT-mix |
Nx 9 |
|
Enzyme mix |
Nx 1.5 |
|
2019-nCoV primer probe |
Nx 1 |
|
Influenza A/B primer probe |
Nx 1 |
|
Total volume |
Nx 12.5 |
Set up
cycle program::
|
Step |
Temp |
Time |
Cycles |
|
1 |
UNG Incubation |
25°C |
2min |
1 |
|
2 |
RT incubation |
50℃ |
10min |
1 |
|
3 |
Enzyme activation |
95℃ |
2min |
1 |
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4 |
Denature |
95℃ |
5sec |
45 |
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Annealing, extension, detction* |
60℃ |
35sec |
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*Fluorescent test in extension:
Channel for 2019-nCoV E gene and N gene is FAM;
Channel for 2019-nCoV ORF1ab gene is ROX;
Channel for influenza A/B is Cy5;
Channel for internal standard is VIC;
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Data
Analysis:
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Ct Value* |
Result |
Recommendations |
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Ct（FAM）≤36.5 and Ct（ROX）≤38 |
2019-nCoV positive(+) |
Active treatment |
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Ct（FAM、ROX）≥39 or no value，and Ct（VIC）＜40 |
2019-nCoV negative(-) |
More Rest |
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Ct（FAM、ROX）≥39 or no value，and Ct（VIC）＜40,but Ct(Cy5) ≤36.5 |
2019-nCoV negative(-)
Influenza A/B positive(+) |
Active treatment |
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Ct（FAM）≤36.5 and Ct（ROX）≤38,and Ct(Cy5) ≤36.5 |
2019-nCoV negative(+)
Influenza A/B positive(+) |
Active treatment |
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only Ct（FAM）≤36.5
or Ct（ROX）≤38 |
Inconclusive Result |
Repeat extraction and double confirm. After getting
Ct（FAM）≤36.5 or Ct（ROX）≤38, the result is
positive |
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36.5＜Ct（FAM）＜39
or 38＜Ct（ROX）＜39 |
Inconclusive Result |
Increase specimen concentration and double confirm.
If Ct（FAM）＜39
and Ct（ROX）＜39,
it means 2019-nCoV result is positive; other results
need be analyzed by other methods. |
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Ct（FAM、ROX、CY5）≥39
or no value，and
Ct（VIC）≥40
or no value |
Invalid Result |
Indicates that there might be issues with experiment
itself, reagent, need check before repeat the
experiment |
SARS-CoV-2(2019-nCOV) Coronavirus and Influenza A/B Virus
Multiplex RT-qPCR Detection Kits
Handbook of COVID-19 Prevention and Treatment
If you want to get bulk order price,please contact us.E-mail:master@shinegene.org.cn or shinegene@vip.163.com
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