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Primers and probes for the detection of the new 2019
coronavirus(Sars-CoV-2) are available from ShineGene!
The new coronavirus
(2019-nCoV) was first detected in Wuhan City, Hubei
Province China. Tens of thousands of infections with
2019-nCoV have been reported in China. The virus is
spreading from person-to-person in parts of the world.
Coronaviruses are a large family of viruses that are
common in many animals, including camels, cattle, cats,
and bats. However, some animal coronaviruses can infect
people and spread between people, for
example MERS, SARS,
and now with 2019-nCoV {CDC}.
Corona virus outbreaks in
January 2020.
Primers and probes recommended by the CDC &
WHO
ShineGene can provide the primer and probe sequences
recommended by the CDC and the WHO to researchers working on
the coronavirus.
The sequences were publically provided by the CDC
here and the WHO
here. Researchers can order these primers and probes to
create a panel in their lab, following the panel
instructions from the CDC.
These materials are provided for
research purposes only.
ShineGene also offer the corresponding
positive
plasmid.
|
Name |
Country |
Label 5’ |
Oligonucleotide Sequence (5’>3’) |
Label 3’ |
Position |
Working Conc. [μM] |
|
|
|
|
N Protein |
|
|
|
|
2019-nCoV_N1-F |
USA |
|
GACCCCAAAATCAGCGAAAT |
|
28287-28306 |
20 |
|
2019-nCoV_N1-R |
|
TCTGGTTACTGCCAGTTGAATCTG |
|
28335-28358 |
20 |
|
2019-nCoV_N1-P |
FAM |
ACCCCGCATTACGTTTGGTGGACC |
BQ1 |
28309--28332 |
5 |
|
2019-nCoV_N2-F |
|
TTACAAACATTGGCCGCAAA |
|
29164-29183 |
20 |
|
2019-nCoV_N2-R |
|
GCGCGACATTCCGAAGAA |
|
29213-29230 |
20 |
|
2019-nCoV_N2-P |
FAM |
ACAATTTGCCCCCAGCGCTTCAG |
BQ1 |
29188--29210 |
5 |
|
2019-nCoV_N3-F |
|
GGGAGCCTTGAATACACCAAAA |
|
28681-28702 |
20 |
|
2019-nCoV_N3-R |
|
TGTAGCACGATTGCAGCATTG |
|
28732-28752 |
20 |
|
2019-nCoV_N3-P |
FAM |
AYCACATTGGCACCCGCAATCCTG |
BQ1 |
28706-28727 |
5 |
|
2019-nCoV-NF |
China |
|
GGGGAACTTCTCCTGCTAGAAT |
|
28881-28902 |
0.6 |
|
2019-nCoV-NR |
|
CAGACATTTTGCTCTCAAGCTG |
|
28958-28979 |
0.8 |
|
2019-nCoV-NP |
FAM |
TTGCTGCTGCTTGACAGATT |
TAMRA |
28934-28953 |
0.1 |
|
HKU-NF |
Hong Kong |
|
TAATCAGACAAGGAACTGATTA |
|
29145-29166 |
0.6 |
|
HKU-NR |
|
CGAAGGTGTGACTTCCATG |
|
29236-29254 |
0.8 |
|
HKU-NP |
FAM |
GCAAATTGTGCAATTTGCGG |
TAMRA |
29196-29177 |
0.1 |
|
NIID_2019-nCOV_N_F2 |
Japanese |
|
AAATTTTGGGGACCAGGAAC |
|
29142-29161 |
0.5 |
|
NIID_2019-nCOV_N_R2 |
|
TGGCAGCTGTGTAGGTCAAC |
|
29280-29299 |
0.7 |
|
NIID_2019-nCOV_N_P2 |
FAM |
ATGTCGCGCATTGGCATGGA |
BQ1 |
29239-29258 |
0.2 |
|
WH-NIC N-F |
Thailand |
|
CGTTTGGTGGACCCTCAGAT |
|
28320-28339 |
0.4 |
|
WH-NIC N-R |
|
CCCCACTGCGTTCTCCATT |
|
28358-28376 |
0.4 |
|
WH-NIC N-P |
FAM |
CAACTGGCAGTAACCA |
MGB |
28341-38356 |
0.1 |
|
|
|
|
E gene |
|
|
|
|
E_Sarbeco_F1 |
Germany |
|
ACAGGTACGTTAATAGTTAATAGCGT |
|
26269-26294 |
0.4 |
|
E_Sarbeco_R2 |
|
ATATTGCAGCAGTACGCACACA |
|
26360-26381 |
0.4 |
|
E_Sarbeco_P1 |
FAM |
ACACTAGCCATCCTTACTGCGCTTCG |
BQ1 |
26332-26357 |
0.2 |
|
CoV-EFP |
China |
|
ACTTCTTTTTCTTGCTTTCGTGGT |
|
26295-26318 |
20 |
|
CoV-ERP |
|
GCAGCAGTACGCACACAATC |
|
26357-26376 |
20 |
|
CoV-EP |
FAM |
CTAGTTACACTAGCCATCCTTACTGC |
BQ1 |
26326-26351 |
5 |
|
|
|
|
ORF1ab |
|
|
|
|
2019-nCoV-OFP |
China |
|
CCCTGTGGGTTTTACACTTAA |
|
13342-13362 |
0.6 |
|
2019-nCoV-ORP |
|
ACGATTGTGCATCAGCTGA |
|
13442-13460 |
0.8 |
|
2019-nCoV-OP |
FAM |
CCGTCTGCGGTATGTGGAAAGGTTATGG |
BQ1 |
13377--13404 |
0.1 |
|
HKU-ORF1b-nsp14F |
Hong Kong |
|
TGGGGYTTTACRGGTAACCT |
|
18778-18797 |
0.6 |
|
HKU- ORF1b-nsp14R |
|
AACRCGCTTAACAAAGCACTC |
|
18889-18909 |
0.8 |
|
HKU-ORF1b-nsp141P |
FAM |
TAGTTGTGATGCWATCATGACTAG |
TAMRA |
18849--18872 |
0.1 |
|
RdRP_SARSr-F2 |
Germany |
|
GTGARATGGTCATGTGTGGCGG |
|
15431-15452 |
0.6 |
|
RdRP_SARSr-R1 |
|
CARATGTTAAASACACTATTAGCATA |
|
15435-15460 |
0.8 |
|
RdRP_SARSr-P2 |
FAM |
CAGGTGGAACCTCATCAGGAGATGC |
BQ1 |
15470-15494 |
0.1 |
|
Name |
|
Label 5’ |
Oligonucleotide Sequence (5’>3’) |
Label 3’ |
|
Working Conc. [μM] |
|
Internal standard |
|
|
RNAse Protein/NM_006413.5 |
|
|
|
|
RP-F |
Recognized |
|
AGATTTGGACCTGCGAGCG |
|
28-46 |
20 |
|
RP-R |
|
GAGCGGCTGTCTCCACAAGT |
|
73-92 |
20 |
|
RP-P RNAse P |
FAM |
TTCTGACCTGAAGGCTCTGCGCG |
BQ1 |
49-71 |
5 |
Note:
In order to prevent any cross-contamination of
primers and probes with control plasmids, our standard
procedure is to spatially separated our production of oligos
and genes/plasmisds by separate rooms and air systems!
Publications
1.(2020) 2019
Novel Coronavirus (2019-nCoV), Wuhan, China. [Online] US
Centers for Disease Control and Prevention. [Accessed 28 Jan
2020].
2.(2020) 2019-Novel
coronavirus (2019-nCoV) real-time rRT-PCR panel primers and
probes. US Centers for
Disease Control and Prevention. [Accessed 28 Jan 2020].
3.(2020) Real-time
RT-PCR panel for detection 2019-novel coronavirus. US
Centers for Disease Control and Prevention. [Accessed 28 Jan
2020].
4.(2020) Emergency
use authorization. [Online] US Centers for Disease
Control and Prevention. [Accessed 28 Jan 2020].
If you want to get bulk order price,please contact us.E-mail:master@shinegene.org.cn or shinegene@vip.163.com
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dNTP
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