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Heat-labile UNG
Catalog # |
Pack size |
Price(USD) |
ZPH0502-S |
1000U(1U/ul) |
$300.00 |
|
ZPH0502-M |
10000U(1U/ul) |
$2700.00 |
ZPH0502-L |
50000U(1U/ul) |
$11000.00 |
Description
Heat-labile contains the equally named enzyme found in the marinebacterium.It
selectively degrades uracil-containing PCR products. After
performing PCR or RT-PCR using dUTP instead of dTTP, PCR
products remain intact after treatment with Heat Labile
Uracil–DNA Glycosylase, whereas contaminating DNA (i.e., not
amplified) is degraded. Heat Labile Uracil–DNA Glycosylase
is completely and irreversibly inactivated by moderate heat
treatment at 50°C for 2min, allowing contamination control
in RT-qPCR. The enzyme hydrolyses the N-glycosylic bond
between the deoxyribose sugar and the base in uracil-containing
DNA leaving an abasic (apyrimidinic) site in DNA but does
not modify uracils in RNA.
Heat Labile Uracil–DNA Glycosylase is
highly active at 20–40°C. No cofactors or divalent cations
are required for activity, and the enzyme is active in most
PCR and RT-PCR buffers. Although the enzyme is active a pH
6.5–9.0, the optimal pH 7.5 is in 50 mM NaCl.
Features:
l
UNG activity at 20-40°C
l
Inactivated at 50°C 2min
Application:
l
RT-qPCR、multiplex
RT-qPCR
l
LAMP
Heat
lablile UNG
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