Subcloning
is a technique used to move a particular gene of
interest from a original vector to a destination vector
in order to further study its functionality,especially
in protein expression.
ShineGene’s subcloning procedure also provides gene
manipulation including tag fusion, cleavage site
insertion. If our clients do not have a gene in hand, we
can clone the gene based on the accession number our
client provided.
Human
cDNA Clone
To order,
please provide:
1. Desired target gene sequence, cloning vector and cloning
sites.
2. The template and its sequence information,Chromas
sequence.
3. If the starting material is plasmid, a 1-2 µg template
plasmid droped on filter paper.
A sufficient amount of culture if starting material is
bacterial culture.
4. Maps, antibiotic resistance, and cloning sites of
destination vectors.
Email:master@shinegene.org.cn
or
shinegene@vip.163.com
Subcloning
Service Order Form
ShineGene
will
1. Perform PCR cloning, subcloning or mutagenesis to get the
desired target construct.
2. Deliver about 1-4ug of plasmid with sequence
chromatograms(electronic) covering your mutation. All
constructs are confirmed by DNA sequencing.
3.The turnaround is about 14 working days depending on the
complexity of the mutagenesis project.
Subcloning/PCR cloning Price:
For
gene less than 1kb,
Eur120.00 /site
For
gene more than 1kb,
Eur150.00/site
Related Link......
Protein Expression
Make Antibody
Pfu DNA Polymerase
Taq DNA Polymerase
siRNA Construction
DNA Sequencing
Peptide Synthesis
Chromas
Lab Consumables
gene synthesis
Mutagenesis
