Oligo Synthesis               

ShineGene has synthesized millions of high-quality oligonucleotides for researchers since it was founded in 2003. The oligos have been applied in almost all fields of molecular biology, and have become the top choice for researchers.ShineGene offer you an innovative line of custom oligonucleotides for your research. As always, every custom DNA oligo is deprotected and desalted – quality is always guaranteed!
To meet the increasing market demand, we have introduced the most advanced high-throughput synthesizers, which provide computer-controlled oligo synthesis, cleavage, and deprotection in a parallel-processing format. Our oligos are synthesized with a typical coupling efficiency of 99% and purified by OPC free of charge. PAGE and HPLC purification are also available with additional charge.

SARS-CoV-2 Probes 
 

Note:Estimate 1 OD=5 nmols=33 µg,for a 20 base oligo          Shipping is extra.Freight is €35.00
Scale	Purification Type	Yield(OD)
40nmol	OPC	2-10 OD
	Page	1-5 OD
	HPLC	1~2 OD
200nmol	OPC	5-20 OD
	Page	2-8OD
	HPLC	1-5OD
1000nmol	OPC	40-80 OD
	Page	20-40 OD
	HPLC	1-40 OD

Oligo Synthesis Order Form  
Standard Oligos

Yield	Price(US$/base)	Working days
2 OD	0.2	2~3days
5 OD	0.23
10 OD	0.4
30 OD	0.9
60 OD	1.65
100 OD	2.7
The price is only for oligos shorter than 60 mer.OPC purified.

Probes for Quantitative, Real-time PCR
 

Taqman probes	2 OD (US$)	5 OD (US$)	10 OD (US$)
Taqman probes with a Black Hole Quencher
5'FAM and 3'BHQ-1	161	225	322
5' HEX and 3' BHQ-1	193	257	386
5' Vic and 3' BHQ-1	300	400	550
5' TET and 3' BHQ-1	193	257	386
5' Cy3 and 3' BHQ-2	185	249	370
5' Cy5 and 3' BHQ-2	242	306	484
5' NED and 3' BHQ-2	300	400	550
5' Joe and 3' BHQ-1	242	306	484
5' TAMRA and 3' BHQ-2	161	225	322
5’-CAL Fluor Red 610 and 3’ BHQ-2	322	386	644
5’-Quasar 670 and 3’ BHQ-2	322	386	644
5’-Quasar 570 and 3’ BHQ-2	403	467	806
Taqman probes with a TAMRA Quencher
5' FAM and 3' TAMRA	145	209	290
5' HEX and 3' TAMRA	161	225	322
5' TET and 3' TAMRA	161	225	322
Turn-around time is about 5-7 working days

 

Molecular Beacon	2 OD (US$)	5 OD (US$)	10 OD (US$)
5'-FAM and 3' Dabcyl	193	257	386
5'-HEX and 3' Dabcyl	241	305	482
5'-TET and 3' Dabcyl	241	305	482
5'-CY3 and 3' Dabcyl	300	364	600
5'-CY5 and 3' Dabcyl	300	364	600
5'-TAMRA and 3' Dabcyl	193	257	386
Turn-around time is about 5-7 working days

 

TaqMan MGB PROBE	2 OD (US$)	5 OD (US$)
5'-FAM and 3' MGB	564	1015
5'-VIC and 3' MGB	645	1161
5'-NED and 3' MGB	645	1161
Turn-around time is about 5-7 working days

Fluorescent Oligos
Fluorophores	2 OD (US$)	5 OD (US$)	10 OD (US$)
5'-FAM	64	72	128
5'-HEX	104	128	208
5'-TET	104	128	208
5'-CY3	208	256	416
5'-CY5	208	256	416
5'-TAMRA	104	128	208
3'-Fluorescein	64	72	128
3'-TAMRA	104	128	208
3'-Cy3	208	256	416
3'-Cy5	208	256	416
3'-Dabcyl	104	128	208
3'-ROX	128	144	256
3'-BHQ-1	128	144	256
3'-BHQ-2	128	144	256
3'-BHQ-3	128	144	256
For long oligos (60~100 mer) multiply 1.4 based on the above prices
Turn-around time is about 3-4 working days.
Other labelings are available upon request.
Oligo Purification
	OPC	PAGE (US$/oligo)	HPLC (US$/oligo)
2 OD	Free	20	20
5 OD	Free	30	30
10 OD	Free	60	60
30 OD	Free	150	150
50 OD	Free	180	180
100 OD	Free	240	240
OPC purification is only for < 60 mer standard oligos and S-oligos.
Long oligos (≥ 60 mer) should be PAGE purified.
Modified oligos should be PAGE or HPLC purified.

Oligo Synthesis Order Form    

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